Composite

Part:BBa_K592017:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)


B0034-ccaS-B0034-ccaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 624
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2711
    Illegal XhoI site found at 2270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1288
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SYnechocystis sp. codon usage not tuned for expression in E.coli. Strong RBS to ensure expression.


Source

CcaR and CcaS can be found in the genome of Synechocystis sp. PCC6803.

References